Lester A. Reinke

Contact Information:
Lester A. Reinke



 

Reinke, Lester A. (HSC)
Professor

Department of:

College Of Pharmacy/Pharmaceutical Sciences -Professor

Center of:

Education:

University of Nebraska Medical Center   Ph.D., (Biomedicinal Chemistry)   1977

Professional Interest/Expertise/Specializations:
Alcoholic liver injury: Although a relationship between chronic alcohol abuse and liver injury has been known for centuries, the mechanisms through which alcohol damages the liver are not clearly established. Alcohol-induced oxidative stress is thought to have a major role in this toxicity. Chronic alcohol exposure induces several forms of hepatic cytochrome P-450, especially CYP 2E1, and this isozyme is considered to be an important source of oxygen radicals. Alcohol also promotes absorption of bacterial endotoxin from the gastrointestinal tract, which activates liver macrophages to increase production of nitric oxide and several inflammatory cytokines. These and related changes in liver function are the major focus of research in my laboratory. Free Radicals and Spin Trapping: Free radicals have been implicated in the pathogenesis of a number of disease states. Because most free radicals are highly reactive and have extremely short half-lives, their roles in tissue injury have been difficult to study directly. Many radicals can be trapped with suitable spin trapping agents, and the resulting spin adducts can be detected with electron paramagnetic resonance (EPR) spectroscopy. My laboratory is exploring novel biological applications of the spin trapping technique, and its potential for the study of free radical toxicology.

Websites:

Dr. Reinke website profile

Research Projects:

Selected Publications:

Differential expression of liver interleukin-6 receptor-alpha in female versus male ethanol-consuming rats.
   Gallucci R.M., Sloan D.K., O'Dell S.J. and Reinke L.A.
Alcohol Clin Exp Res 28: 365-373
Detection of alfatoxin-induced lipid free radicals in rat bile.
   Towner, R.A., Mason, R.P. and Reinke, L.A.
Biochim Biophys Acta 1573: 55-62
Substituent effect on the rate of the hydroxyl and phenyl radical spin trapping with nitrones.
   Sueishi, Y., Yoshioka, C., Olea-Azar, C., Reinke, L.A. and Kotake, Y.
Bull Chem Soc Jpn 75: 2043-2047
Spin trapping evidence for alcohol-associated oxidative stress.
   Reinke L.A.
Free Rad. Biol. Aging 32: 953-957
Estrogen Suppresses Hepatic IkB Expression During Short-term Alcohol Exposure
   Lee EG, Mickle-Kawar BM, Reinke LA, Gallucci RM
Inflamm. Res.
NF-kB activation is associated with free radical generation and endotoxemia, and precedes pathological liver injury in experimental alcoholic liver disease.
   Jokelainen K, Reinke LA and Nanji AA
Cytokine 16: 36-39, 2001.
Antioxidants and gadolinium chloride attenuate hepatic parenchymal and endothelial cell injury induced by low flow ischemia and reperfusion in perfused rat livers.
   Bailey SM and Reinke LA
Free Rad. Res. 32: 497-506 (2000)
Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats.
   Reinke LA, Moore DR and Nanji AA
Alcohol. Clin. Exp. Res. 24: 332-335 (2000).
Continuous monitoring of in vivo nitric oxide formation using EPR analysis in biliary flow.
   Kotake Y, Moore DR, Sang H and Reinke LA
Nitric Oxide Biol. Chem. 3: 114-122 (1999).
Free radical generation in the cochlea during combined exposure to noise and carbon monoxide: an electrophysiological and an EPR study.
   Rao D., Moore D.R., Reinke, L.A. and Fechter, L.D.
Hearing Res. 161: 113-122
Keywords, Tags:
hepatotoxicity, alcohol, liver,