Muayyad R. Al-ubaidi

Contact Information:
Muayyad R. Al-ubaidi



 

Al-ubaidi, Muayyad R. (HSC)
Professor,Vice Chair of Research

Department of:

College Of Medicine/Cell Biology -Professor, Vice Chair of Research

Center of:

OK Center for Neurosciences -Professor

Education:

Baylor College of Medicine   PhD   1987

Professional Interest/Expertise/Specializations:
Extracellular matrix and retinal homeostasis. This research is to investigate how the extracellular matrix (ECM) supports retinal health and determine its contribution to the degenerative process in inherited retinal diseases. To study retinal ECM, we focused on tyrosine sulfated proteins. Tyrosine sulfation is a post-translational modification that occurs only on secreted and membrane proteins. It is catalyzed by two trans-Golgi tyrosine sulfotransferases (TPST-1 and TPST-2). Retinal function in Tpst1-/-, Tpst2-/- and double knockout (DKO) mice was evaluated and showed that the DKO animals exhibit drastic functional reduction associated with abnormal photoreceptor ultrastructure. This demonstrated the role ECM plays in retinal function. Glycosylation of rhodopsin (RHO). RHO is glycosylated at Asn-2 and 15 on its N-terminus. To understand the role of RHO¿s glycosylated N-terminus, two transgenic mouse models were generated. The No-glycosylation (NOG) model expresses a mutant form of RHO that is not glycosylated at all. NOG mice exhibited a dominant form of retinal degeneration. Electron microscopic examination at postnatal day 30 revealed the presence of vacuolar structures that partially distorted the rod photoreceptor outer segment. Degeneration progressed with age, and by P120 the animals lost ~90% of their retinal function. When bred into the Rho-/- background, we found that the protein appears unstable and is regulated by ubiquitin-mediated proteasomal degradation. The second transgenic mouse model is to investigate the role of the entire N-terminus in the function of RHO. Transgenic mice were generated that expresses a RHO-Beta-3 adrenergic (ß3AR) chimeric protein, designated NBC. The NBC protein contains the body of the ß3AR as well as the N- and C-termini of RHO. Establishment and subsequent characterization of the NBC model in the Rho-/- genetic background revealed that the glycosylated N-terminus of RHO is sufficient for OS morphogenesis.

Websites:

Research Projects:

Selected Publications:

p53 selectively regulates developmental apoptosis of rod photoreceptors.
2013    Vuong, L., Brobst, D.E., Ivanovic, I., Sherry, D.M., Al-Ubaidi1, M.R.
PLoS One 8:e67381
Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
2012    Sherry, D.M., Kanan, Y., Hamilton, R., Hoffhines, A., Arbogast, K.L., Fliesler, S.J., Naash, M.I., Moore, K.L. and Al-Ubaidi, M.R.
PLoS One 7:e39702.
Expression and role of p53 in the retina.
2012    Vuong, L., Conley, S.M. and Al-Ubaidi, M.R.
Invest. Ophthal. Vis. Sci. 53; 1362-71.
Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
2012    Sherry, D.M., Kanan, Y., Hamilton, R., Hoffhines, A., Arbogast, K.L., Fliesler, S.J., Naash, M.I., Moore, K.L. and Al-Ubaidi, M.R.
PLoS One 7:e39702.
Sulfotyrosine
2012    Kanan, Y., Hamilton, R., Sherry, D.M., and Al-Ubaidi, M.R.
Focus on Molecules, Experimental Eye Research 105; 85-86.
Protein tyrosine-O-sulfation in bovine ocular tissues.
2012    Kanan Y., Hamilton R.A., Moore K.L., Al-Ubaidi M.R.
Adv. Exp. Med. Biol. 723; 835-41.
Analysis of genes differentially expressed during retinal degeneration in three mouse models.
2010    Kanan, Y., Centola, M., Bart, F., Al-Ubaidi, M.R.
Adv. Exp. Med. Biol. 664:3-13.
Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.
2010    Sherry, D.M., Murray, A.R., Kanan, Y., Arbogast, K.L., Hamilton, R.A., Fliesler, S.J., Burns, M.E., Moore, K.L. and Al-Ubaidi, M.R.
European Journal of Neuroscience 32: 1461-72.
Protein tyrosine-O-sulfation in the retina.
2009    Kanan,Y., Hoffhines, A., Rauhauser, A., Murray, A., and Al-Ubaidi, M.R.
Exp. Eye Res. 89; 559-67
Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment.
   Kanan, Y., Brobst, D. Westmuckett, A.D., Moore, K.L., Han,Z., Naash, M.I. Al-Ubaidi, M.R.
J. Biol. Chem. In press.